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wga staining solution  (Biotium)


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    Structured Review

    Biotium wga staining solution
    a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, <t>mounting,</t> <t>staining,</t> and image acquisition. b Representative images of <t>WGA-stained</t> lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.
    Wga Staining Solution, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wga+staining+solution/pmc11799539-191-14-17?v=Biotium
    Average 96 stars, based on 446 article reviews
    wga staining solution - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma"

    Article Title: Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma

    Journal: NPJ Precision Oncology

    doi: 10.1038/s41698-025-00819-7

    a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.
    Figure Legend Snippet: a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.

    Techniques Used: Staining, Laser Capture Microdissection, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy



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    Image Search Results


    a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.

    Journal: NPJ Precision Oncology

    Article Title: Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma

    doi: 10.1038/s41698-025-00819-7

    Figure Lengend Snippet: a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.

    Article Snippet: For Wheat Germ Agglutinin (WGA) labeling, sections on membrane PEN slides were incubated with WGA staining solution (Biotium, 29023; diluted 1:1000) in a light-protected environment at 37 °C for 10 min. For pan-cytokeratin (CK), CD8, PD1, PDL1 and pATR staining, antigen retrieval was achieved by immersing the tissue sections on glass slides in EDTA buffer (Sigma, E1161; pH 8.5) at 90 °C for 30 min.

    Techniques: Staining, Laser Capture Microdissection, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy